Google is once more number one search engine in the U.S. for the month of June with a market share of 65%, research firm Nielsen said in a report.

Google’s cut of the U.S. search pie was 65% or almost 6 billion queries out of the total 9.1 billion conducted in June, just 0.1% below May’s level and compared to a 66.1% share a year ago.

Yahoo came in number two. It registered 1.2 billion queries, or 13.7% of the total market, or the same slight 0.1% weaker month-on-month performance but a more significant drop from 16.2% the same period last year.

Closing in on the gap with Yahoo, number three was MSN/Windows Live/Bing, accounting for 13.4% of the U.S. search market in June, up 0.4% from last month and also a jump from last year’s 8.9%.

Number four was AOL at 2.1%, unchanged from last month and down from 3% last year. Ask.com was number five, at 2%, also unchanged from May but up from 1.8% in June 2009.

Google, Yahoo and the Microsoft sites accounted for 92% of total U.S. searches in June, compared with 91.8% in May. Including AOL and Ask.com, the top five search engines accounted for 96.1% of total U.S. queries in June, up from 95.9% in May.

Nielsen said it does not count contextual searches.

Google is slated to hold its second-quarter earnings call http://investor.google.com/earnings.html tomorrow, July 15th, at 1.30 PM PST or 4.30 PM EST.

UPDATE: figures corrected from million to billion

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